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Leica DM LB Microscope

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Leica DM LB 100T

Contact Information

www.leica.com Faculty Contact: Aaron Hawkins
Maintenance Request Staff Contact: Jim Fraser
General Information
Equipment Specifications
Operating Procedure

The Leica DM LB is the premiere optical microscope in the BYU Cleanroom. Its high resolution, precision imaging allows its users to get an accurate, detailed view of their work. The Leica is also equipped with a video camera and computer linkup which makes capturing and sharing the microscopes images simple.

Microscope Lens Calibration

LENS_________DISTANCE BETWEEN NUMBER MARKS
X10_________________123 microns
X20_________________60 microns
X50_________________24 microns
X100________________12 microns

Function Instructions

Power, Brightness, Focus
Stage Movement
Aperture Diaphragm
Field Diaphragm

Aperture Diaphragm

The aperture diaphragm can be narrowed to decrease the amount of light hitting the specimen. This will decrease reflections off the specimen and increase the image contrast. However, closing the aperture too much will cause a loss in detail.

Field Diaphragm

The field diaphragm can be narrowed to decrease the field of view.

Polarizer

Capturing Images on the Computer Turn on the camera by flipping the switch on the black box behind the microscope. (the green light will turn on on top of the camera) Click on the FBG32 icon on the computer desktop.

In the program the menu tab furthest right will read either GRAB or LIVE. If it says GRAB the program is showing the live feed from the camera and the user has the option of grabbing the current image. It it says LIVE, the program is showing the last image grabbed and the user can press live to see the live feed from the camera. Save option is under the file tab.